Dear , you will find useful information in links below:

onlinelibrary.wiley.com/doi/10.1111/evj.12171/pdf

https://www.zoetisus.com/_.../eqb12074_effectmetastim_techbulletin_102312_final.p...

www.offlu.net/index.php?id=345

Hello

This protocol can be helpful:

Addition of one-half volume of phosphate saline containing Tween 80 to 1 volume of simple water in oil/oil in water emulsion. The mixture was then re-emulsified to produce a double emulsion.

Generally, the more nanoparticulated one from Montanide adjuvant will be better.

Dear Batmagnai Enkhbaatar;

I salute you!

Two alternatives:

1)The Montanide serie consists of certain number of good adjuvants.the best practice is to make an experiment with small batches of the vaccine and compare immunogenicity,side reactions,mixture complications and costs,and decide!!!

2) I have experience developing my own nanoparticulate formulation without montanide,using soy fosfolipids,but i would need to know more details about your antigens.

Sincerely yours

Gustavo

Dear Gustavo, I just weonderin about Soy phospholipid, it is similar to those we use for liposome fabrication?gand they need to use cholestrole or not?

Dear Elrashdy M Redwan ,SALUD!!!

Depending upon the antigen,and general formulation you are going to mixture,cholesterol is needed or not. Very frecuently,if your mixture contains lipids able to form with soy fosfolipids stable liposomes,after that you may not need the chlesterol addition!

Sincerely yours!

Gustavo

Elrashdy M Redwan

I dont have had feed back about the immunogen preparation with orr without colesterol!!!??? And or fosfolipids!???

Gustavo