How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to extract and purify disaccharide peptides and lactoyl peptides from Mycobacteria cell wall?

Recently, I try to obtain disaccharide peptides and lactoyl peptides from Mycobacterium Smegmatis, but I cannot succeed. My method is: Disaccharide peptides: The mycobacterial strains were...

12 January 2021 8,352 3 View

QTOF vs Orbitrap for CECs?

We considering purchase of Sciex QTOF and Thermo Exploris 120 MS for unknown compound screen (CECs), id, and quantification in water matrix, at low conc. etc. Which one would be most...

06 January 2021 4,931 1 View

Could anyone professional help me to solve this problem?

I have installed the software BRUKER Flexanalysis 3.4 on my computer successfully. But when I tried to import the MS data produced by BRUKER MALDI-TOF-MS, I met with an error described in the...

10 November 2020 8,205 1 View

Best way to add streptavidin onto flat surface?

I will be adding streptavidin onto the surface of a graphene coated glass slide; thereafter, I will be analyzing with a MALDI-TOF. I was wondering the best way to go about this? I was thinking of...

07 September 2020 5,902 4 View

Why do I get multiple peaks of my protein when measuring molecular weight using MALDI-TOF?

I have gotten the MALDI-TOF results of my protein (Gliadin from wheat) however it shows different peaks. What does it say about my protein? (see readings attached) I need to know the total...

27 July 2020 1,074 1 View

Flex analysis Maldi-TOF file error?

I am receiving an error message suggesting that the fid file might be the wrong format, when I try to import it into flex analysis. Is there anyway to solve this issue?

12 March 2020 8,707 3 View

Does anyone know about analysis of 3D mapping and depth profile for wooden object?

I have 1cm cubic size wood samples. inside of wood, there are S and S-organic, inorganic compounds randomly. I'd like to figure out the quantities of S distribution non-destructively. First, I...

20 February 2020 9,303 2 View

Can any one have M/Z (mass) values of standard chart for analysing MALDI-TOF?

How to identify unknown compound by the chromatogram and peaks received by MALDI-ToF

17 February 2020 5,390 3 View

What is the procedure of removing an incorrect article from the list of my publications on Research Gate and replacing it with the correct one?

One of my recent articles was erroneously uploaded to Research Gate with incorrect title and as the printer's proof. I would like to delete it and replace it with the file as published by the...

17 December 2019 3,428 1 View