Dear Saiful Anuar Karsani,

The first requirement for database searching is a peak list; you cannot upload a raw data file. Raw data is converted into a peak list by a process called peak picking or peak detection. Often, the instrument data system takes care of this, and you can submit a Mascot search directly from the data system or save a peak list to a disk file for submission using the web browser search form. If not, or if you have a raw data file and no access to the data system, you’ll need to find a utility to convert it into a peak list. Peak lists are text files and come in various different formats. If you have a choice, MGF is recommended. Be careful with mzML, because this may contain either raw data or a peak list.

http://www.matrixscience.com/help/mis_help.html

http://www.matrixscience.com/help/data_file_help.html

Regards, Shafagat