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I ordered two Predesigned assays for quantification of CpG methylation by Pyrosequencing from Qiagen and I do not have any product after PCR. I have dissolved them in TE ph8, tried different...

22 September 2020 3,328 6 View

What does it mean to see a band for uncloned vector and one band for vector with insert from one colony after cloning?

I used gateway cloning to clone my insert into the donor vector. After having colonies, I did a mini prep and ran it on the gel. On the gel I could see a clear band on the right length for my...

14 June 2020 9,829 3 View

What are the soil nutrients use efficiencies in Central America?

Hello everyone, I am working in Central America and I would like to create a document that could help me to calculate "simply" the nutrients disponibility in the soil of coffee fields. The...

10 June 2020 9,671 4 View

How to perform gradient qPCR with Roche Light Cycler 480, to determine the best annealing temperature of my primers??

I would like to determine the best annealing temperature for my primers, giving the lowest Cq and the best efficiency. Is there a thermal gradient feature on the Roche LC480 instrument? And if so,...

16 February 2020 9,471 3 View

Which statistical analysis should I use for 2 dependent variables that are measured with a Likert Scale?

I am studying the generational changes in the relationship between empathy and social media usage. I am using a Likert Scale to measure both empathy levels and social media usage; however, I do...

20 January 2020 9,617 4 View

Is there a way to statistically separate the top percent of performers in a pre-test condition, to then analyse their scores in a post-test measure?

Essentially in a study where there is a pre-test, then an intervention and a post-test (Identical tests), I have a question: "Do students who perform better on the skill test before the...

12 December 2019 9,096 4 View

Why are my re-probings for Western blot analysis unsuccessful?

Hello everyone, I am having problems with re-probing membranes for Western blot analysis. Almost every time I want to re-probe with either the same or a new primary antibody, I don't get any...

11 December 2019 5,066 5 View

How to dissolve and inject 4-hydroxytamoxifen to nude mouse?

What is the normal way to inject 500 ug 4-hydroxytamoxifen per mouse? Is it dissolved 4-OHT in ethanol and diluted with peanut oil at least 10 times?

10 May 2019 3,301 1 View

Why does this strange situation occur in my nude mice xenograft after drug injection?

I injected cancer cells to mice as xenografts. After 7 days, I injected tamoxifen together with an inhibitor of interleukin to nude mice subcutaneously. But 2 days later, the tumor site turned to...

21 March 2019 6,082 4 View

Does anyone have experience in cleaving Fibronectin using an MMP before Western Blot analysis?

We are trying to demonstrate the our treatment effects increases the activity of MMP-2 by showing mutiple cleaved fibronectin bands in a western blot? For this we are going to have a control of...

13 February 2019 7,734 3 View

Which test should I use in SPSS if I have two independent variables and one dependent variable that is measured using two tests?

I am researching the effect of gender and video game use on reaction times. To do this, I will have participants complete two reaction time tests (a simple test and a four choice test). I am a...

17 February 2021 9,343 4 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View

Does anyone know a downloadable dataset for people sitting in front a webcam?

I am working on a ML project that recognize the posture using the webcam. So, i need a dataset of people sitting in front the webcam (studying, gaming, video calling...etc)

26 January 2021 5,652 3 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

How to use PVQ Psychometric Tool?

Hello, I'm doing a research about if psychometric tools can be translated into video games. My main major is video games. So I'm an absolute newbie with psychometric tools. I have chosen PVQ tool...

22 January 2021 5,992 2 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Can't find an ancient zoology paper ?

I'm desperately looking for an old planarian paper which is cited here. Does anyone have an idea how to get hold of it...

21 January 2021 5,957 8 View

Which Method of Statistical Analysis is best suit for making comparisons?

For the purpose of selecting the best one among the 4 design approaches, experimental video surveillance has taken. The dependent variable is the distance (meter) and the independent variables...

21 January 2021 4,936 7 View

Genus name priority from the same publication (Zoology)?

I came across a recent taxonomic work, where two genera were synonymized. Both genera were published in a paper in 1922 by the original author who revised the family at that time. The original...

19 January 2021 1,920 4 View