To study the calcium and sodium transport function of one transporter gene I want to study them in k667 yeast mutant strain. So can anyone guide me me how and where to buy this strain?
Thank you in advance.
You can buy/order from ATCC. There is also GE Dharmacon company that sells yeasts.
http://dharmacon.gelifesciences.com/gene-expression-cdnas-orfs/non-mammalian-cdnas-and-orfs/yeast/
http://www.atcc.org/Products/Cells_and_Microorganisms/Fungi_and_Yeast.aspx
http://www.atcc.org/Products/Cells%20and%20Microorganisms/Fungi%20and%20Yeast/Saccharomyces%20cerevisiae%20Deletion%20Mutants.aspx
Hi Harshraj,
I bet that you have already read this 2015 paper attached, but just in case, I attach it for you. The authors from this paper had used K667 mutant yeast for their research. They asked this mutant from Prof. Kyle Cunningham (see below**), and acknowledged it in the 'Acknowledgements' section of their paper (see attachment, p.14, yellow highlight). If you cannot buy it, you should contact Dr. Cunningham. I don't think that the Indian group (from the attached 2015 paper) can give you K667, although they are in Asia, near Japan. Because, most likely, they have signed 'Material Transfer Agreement' (MTA) form, meaning they cannot give the material to any third party without the consent of the original owner.
**"Prof. Kyle W. Cunningham (Johns Hopkins University, Baltimore, MD, USA) for yeast wild-type K601/W303-1A and triple mutant K667 (∆cnb1∆pmc1∆vcx1) strains."
I browsed through the paper second time, and I saw this message on the first page:
"Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi-110021, India. Correspondence and requests for materials should be addressed to G.K.P. (email: gkpandey@ south.du.ac.in)"
Send an email to G.K.P. regarding the request of K667. If he cannot give the material due to MTA, you should contact Prof. Kyle W. Cunningham.
Thank you Yuan once again for all your efforts. I contacted to Indian group but no response from them. After sending several email i got response from one salinity research center of Harbin (China) they are sending me both k667 yeast mutant and Saccharomyces cerev. 9.3. I am happy to hear from them.
Thank you Hriush Adhikari . Unfortunately this strain is not available in ATCC and Dharmacon.
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