Hi Brenna,

Different companies sell DNAse I for RNA treatment. 

Thermo Fisher: 2,00 units (https://www.thermofisher.com/order/catalog/product/AM2222) or 10,000 units (https://www.thermofisher.com/order/catalog/product/AM2224)

Sigma-aldrich: http://www.sigmaaldrich.com/catalog/substance/deoxyribonucleaseifrombovinepancreas12345900398911?lang=pt®ion=BR

NEB: https://www.neb.com/products/m0303-dnase-i-rnase-free

Qiagen: https://www.qiagen.com/us/shop/sample-technologies/dna/dna-preparation/dnase-max-kit/#orderinginformation

I hope this could help you.

Best,

Michele

Hi Michele, thank you for your reply! Unfortunately I am not looking for DNase, I am needing DNase-free RNase. Need to treat my genomic DNA with RNase to get rid of any contaminating RNA.

Oh sorry Brenna! I read wrong your doubt.

Qiagen: https://www.qiagen.com/us/shop/lab-basics/enzymes/rnase-a/ (I usually use this one)

Sigma: http://www.sigmaaldrich.com/catalog/product/roche/11119915001

Thermo: https://www.thermofisher.com/order/catalog/product/12091021

Others Thermo options: https://www.thermofisher.com/search/results?query=rnase&persona=Catalog&navId=10844

Best,

Michele

Hi Michele, 

For some reason I wasn't alerted that you answered my question! Thank you! I ended up going with the Qiagen RNase A but upon their recommendation I will be heating the enzyme for 15 minutes at 80 degrees and then cooling it to room temperature before use. Qiagen doesn't guarantee their RNase A (100mg/ml) to be DNase free so I will be heating it to denature any possible contaminants.

Thank you!

Brenna