Where can one find temporal transcriptome data for seed germination in Arabidopsis thaliana?

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In untargeted metabolomic (LCMS) the standard is not aligning with all my previous annotation data. What happened except its not a correct annotation?

In untargeted metabolomic (LCMS) my analytical standard is not in the same retention time as my annotation. The annotation was done through isotope-labeling to annotate the elemental formula,...

26 December 2023 1,395 2 View

Error via Link 601, how can I solve it?

I'm using Gaussian 09 to optimize a transition state' but it fails every time with this error: "No NMR shielding tensors so no spin-rotation constants. Leave Link 601 at Tue Apr 11 17:40:06...

27 April 2023 5,090 0 View

Does UV radiation affects plant's hormones (e.g. BAP, IAA...)?

I have radiated the plates with UV in the Lamina Flow hood after pouring an MS + PGR's media to them. Does it ruins the media or lowering any of it's compounds concentrations?

06 April 2022 5,513 10 View

Is anyone familiar with experimental measures of any of the 24 character strengths?

Thank you for your willingness to answer my question! I'm looking for previously used as well as original ways to measure character strengths experimentally. I know it's a very broad question, but...

28 July 2015 2,066 5 View

Sanity checks for extended Kalman filter

I am working on an error state extended Kalman filter for navigation using IMU and DVL. What kind of sanity checks are you using to test your filter?

01 January 1970 6,463 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View