Where can I see a site for putative genes in rice?

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What is the best way for the colony PCR to work efficiently?

I am having a problem in doing colony PCR. Sometimes it works, sometimes it won't. Basically, what I do is to touch the edge of the colony with a toothpick and then submerged it to the PCR...

09 October 2014 3,857 25 View

Are there any methods for efficient isolation of plasmid DNA in Agro that are good for sequencing and transformation?

I am using an Invitrogen Midi prep Kit, however, I always get a very low concentration even though my initial volume is around 10-25ml.

06 July 2014 2,900 3 View

How to isolate a good number of rice protoplast cells to be used in PEG transformation?

I wanted to try the activity of our TALEN constructs in rice protoplast first before we used rice immature embryo for transformation, and I am finding difficulty in isolating good number of...

05 June 2014 8,218 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Christian Cantos

Thank you Kaushal!

Sibtain Afzal

dear Christian

Plz read the below 3 articles zipped together. If you need more articles that u cant download. feel free to contact.

Best Regards

Didier Andrivon

Hi all

You can also have a look at the following database

http://orygenesdb.cirad.fr/

All the best

Didier