Where can I get the T7 bacteriophage gene?

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Panduranga Rao

Hi,

you can try finding it at

http://parts.igem.org/Main_Page

Quick Ida

Hello Panduranga. Very helpful page. But I could only find T7 promoter not T7 RNA polymerase gene.

This plasmid (pTARA) may be useful for you

https://www.addgene.org/31491/

That would be useful. I guess I can try to amplify the gene from pTARA and use it in mammalian expression vector. Thank you Panduranga.

Sandip Chakraborty

You can refer to the following links for getting crucial informations:-

1. http://mol-biol4masters.masters.grkraj.org/html/Prokaryotic_DNA_Replication13-T7_Phage_DNA_Replication.htm

2. http://www.bio.davidson.edu/genomics/method/T7list.html

3. http://www.pnas.org/content/81/7/2035.full.pdf

Thank you Sandip. I guess I need to figure out some modification to express T7 RNAP in mammalian cell line. Those would be very invaluable information.