30 May 2017 2 7K Report

Can anybody refer me where to obtain immortalized human Schwann cells? I am planning on working with them but I am having a hard time obtaining some. Thanks!

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What are the ice water bath conditions for the oxidation synthesis method?

The bulk-quantity 1D PANI nanowire/tubes were prepared as follows: analytical grade aniline (4 mmol) and tartaricacid (1 mmol) were dispersed in 10 mL of deionized water at room temperature with...

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What is Exactly Triangulation in Educational Research?

How can we execute it in different methods of Educational Research

22 October 2023 9,888 28 View

SPRI bead clean up post Indexing PCR Step in NGS Library Generation?

I have been making NGS and WGBS Libraries for a while now and keep noticing an odd trend with my SPRI beads at the Indexing PCR step. When I go to elute my final Library off the SPRI beads, the...

19 June 2023 8,894 3 View

Is ResearchGate now blocked in China?

Until recently I had no problems accessing RG from the mainland, but currently if appears to be blocked. Is it just me or my specific location?

26 February 2023 2,737 4 View

Why is my coin cell not able to hold its charge and discharge after initial first charge?

The practical calculated capacity of my coin cell LiCoO2/graphite is 0.640mAh. The electrolyte used is 1M LiPF6 in 1:1 EC/DEC. Immediately after assembling the cell, I charge at a given current...

17 February 2023 6,324 1 View

Can you identify the genus of this chironomidae larvae from french Guyane (neotropical genus) ?

Hello community, i'm working on Chironomidae (diptera) from french Guyana and i found one that i can't identify because the larvae didn't correspond at any genus already known and present in...

26 January 2023 8,461 4 View

How to couple FSI (Fluid structure interaction) with electric field in COMSOL?

I want to investigate the interaction between an electret (fluttering in the wind) and a nearby electrode. So the fluttering motion is both affected by the flow and electric field between the...

24 November 2022 9,289 0 View

How can i covert sequence data from FASTA to a VCF format?

Hi!, I am trying to figure out the connectivity between subpopulations of a parrotfish species along the coast of western Africa by analyzing the differences of neutral mutations in SNPs in the...

13 July 2022 8,670 3 View

Possible reasons for AKTA Pure 25 MonoQ column giving high fluctuating pressure?

Hi everyone, Over the past few months the pre and delta column pressure when using our monoQ column has steadily been increasing, and now we've had to reduce our flow rate to 1/4 normal rate to...

05 July 2022 1,132 0 View

Way higher than expected Bonding energy of two carboxylic acids, is it normal ?

Hi ! I'm studying a Heme-Heme dimer (see sketch, chemdraw structure is one Heme ring) and I want to compare the energy of before and after complexation of the propionic acid side-chain to an...

12 June 2022 6,720 1 View

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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Do I need specific antibodies to stain for a fusion protein?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

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