Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

Recommendations for IL-10 Receptor staining?

11 October 2020 9,412 3 View

What is appropriate to use as my affiliation for a manuscript after finishing graduate school?

I would like to publish my graduate (M.S.) research, which is wildlife/conservation-related, and which I conducted at a U.S. university. It's been a few years since I finished graduate school. I...

14 September 2020 6,153 19 View

Do you mind answering a quick survey ? One minute only ?

Hi How are you ? languages are important in our world.. Mostly for you, if you need us to proofreading your articles. You need good translators to do so.. I set up my translation agency by the...

23 May 2020 1,323 3 View

What marker can I use to analyze dendritic spine maturity on a tissue section?

I would like to quantify the number of dendritic spines in all the maturation stages by using a coronal section of a mouse brain. I will be looking more specifically in the hippocampus.

30 September 2019 7,816 5 View

How do I write a PDB file with custom non-atom particles for a coarse grain model for molecular dynamics simulations (on GROMACS)?

I am trying to write custom particles to a PDB file for coarse grain molecular dynamic simulations in GROMACS. In particular, I am trying to model RNA nucleotides using a handful of beads per...

05 July 2018 7,785 2 View

Uninvited guest in intestinal organoids?

Please excuse the poor photograph. This very large body has appeared in my mouse intestinal organoid culture. To the right (out of focus) is my budding organoid, and in the centre is this very...

14 February 2017 6,791 2 View

What causes huge black dots on (nitrocellulose) membranes?

Dear all, I transferred proteins labeled with a fluorescent dye from (a part of) a 2D SDS-PAGE gel to a nitrocellulose membrane. After scanning the nitrocellulose membrane to check whether the...

27 October 2016 2,816 3 View

Is silver citrate hydrate and/or silver acetylacetonate soluble in ethanol?

For a synthesis I need a silver derivative that is soluble in ethanol. Does anyone have any experience on how well silver citrate hydrate or silver acetylacetonate dissolve in ethanol? It is...

28 July 2016 1,376 3 View

What is the best method to avoid contamination between samples that need to be homogenized prior to DNA extraction?

We have many samples to be homogenized and the material need to be washed between samples to avoid DNA contamination between them. It is hard to effectively bleach all the materials. I did some...

01 May 2016 443 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

How to perform CV by using potentiostat?

I have deposited a metal oxide thin film of glass plate, A conductive thin film was also deposited on the glass substrate before the deposition of metal oxide film. How can I perform its CV from a...

01 February 2021 7,710 3 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

What parameters of quality control would I have to consider for antimicrobial toothpaste?

Hello, my name is Bianca and as a project for university I have assigned the manufacturing of antimicrobial toothpaste- quality control department. I tried to research online but I could not find...

17 January 2021 918 3 View

I am asking about the drug release from minicells, to test the antimicrobial activity of the drug?

I am working on minicells loaded with the drug, I want to test the antimicrobial activity of the drug loaded in minicells using agar diffusion method, many trials didn't produce any zone of...

17 January 2021 7,511 3 View

How do you determine at what concentration of antimicrobial should you subject the pathogen to?

I am making an antimicrobial extract from fungal metabolite but the fungi are not known. So I don't know at what concentration I should subject them to but what I do know I will test them against...

07 January 2021 897 6 View

Which antimicrobial test will be suitable to determine antimicrobial activity of silver nanoparticles?

There are some journal articles that reported well diffusion method to determine the antimicrobial activity of silver nanoparticles. Meanwhile, some reported disc diffusion method. Which method is...

06 January 2021 7,123 4 View

How yo prepare samples for RNA extraction?

I really need help. I tried finding a protocol on how to prepare samples for gene expression . I am working on Antimicrobial activity of a medicinal plant and I would like to check its effect on...

30 December 2020 7,112 2 View