Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

How to perform cell transfection and Dual Glo assay with low-adhesive (HEK293) cells?

Hello everyone. I need to use Promega Dual Glo assay for HEK293 cells, but I am really afraid of wash out my cells from the 96-well plate, and at the same time, I am afraid to use coating because...

09 September 2020 4,817 7 View

How to reduce auto fluorescence in bone-marrow smears that would be used for FISH and IHC?

We are processing clinical samples of bone marrow smears, and have encountered autofluorescence that makes subsequent FISH and IHC virtually impossible. The problems are: 1. the samples are of...

18 December 2019 4,676 4 View

Antibody detects protein which was ablated in null mice (global knockout). Anyone had similar experience?

Here is a dilemma: there is a line of null mice (global knockout), which are null by genotyping and by mRNA analysis (qPCR). They were generated by replacement of an entire protein-coding region...

16 March 2019 9,722 6 View

Does high fidelity taq polymerase get less efficient after expiration date?

24 September 2016 5,468 4 View

Wrong or no fragment insert in directional TOPO cloning?

17 July 2016 10,020 10 View

Why in the analysis of cyanide, ninhydrin dissolved in sodium carbonate?

I was doing research related to the analysis of cyanide with ninhydrin, in some journals mentioned that preparation ninhydrin by dissolving it in sodium carbonate, but I do not understand why. Is...

12 July 2016 8,541 4 View

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15 June 2016 5,924 6 View

Why do I get a wrong length of PCR result but after sequencing it is actually a right target sequence?

Can somebody help me? I am doing a PCR of worm tissue. I used KAPA Taq Extra HotStart ReadyMix. I should be getting a PCR result of 158bp but I always get a 200 bp PCR result and after I sequence...

15 June 2016 7,313 6 View

What are the causes of PCR failure? i change the PCR master mix, optimized the annealing temperature and so on. but still no defined product band?

i want to amplified Channa striata COI gene using universal fish primer, out of 13 samples only two showed defined product bands after several optimization. I change the PCR mix, change the...

06 March 2016 854 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View