Where can I find a good protocol to prepare competent E.coli cells with CaCl2 in lab?

Barbara Mandriani @Barbara-Mandriani

26 February 2025 0 2K Report

I am looking for a standardized protocol to prepare calcium chloride-competent E.coli cells.

Thanks

Badges
Science topic

Similar topics
Agriculture
Horticulture
Seeds

More Barbara Mandriani's questions See All

Who is writing about the similarities and differences of Judaism and Ubuntu?

seeking research on similarities and differences between Ubuntu and Judaism similarities between Levinas and Judaism

21 July 2024 8,545 0 View

MS/MS spectrum with 5 Da difference in m/z value?

Hi everyone, We experienced a strange pattern in some spectra coming from HRMS/MS analysis. In particular, in the MS1 spectrum a base peak at m/z=M and a smaller peak at m/z=M+5Da appear, but in...

08 July 2024 3,631 4 View

I am researching for materials pertaining to introducing the nursing field to school age children to spark interest in nursing profession /healthcare?

Hello I am researching this topic to obtain resources to write a paper at our facility to reinforce the need to have involvement with the local schools for a topic close to my heart nursing....

11 June 2024 6,634 5 View

Doing research on foresight and technological innovation?

If you're doing research on foresight and/or technological development and diffusion, then you might want to consider this call for abstracts. It's for a special issue of Wiley's Futures and...

02 April 2024 3,841 0 View

Plus-value de visites à domicile par l'équipe de diabétologie pédiatrique dans le parcours de soins d'enfants porteurs d'un diabète type 1?

Etudiante en Master 1 infirmière ne pratique avancée, je réalise une revue de littérature sur cette question de départ pour en extraire une question de recherche

26 March 2024 3,081 3 View

Does anyone know the general costs of a third generation Pacbio sequencer, in materials, reagents, etc?

Any information about this?

20 March 2024 1,475 0 View

Who are the most read authors on the use of focus group discussion as a qualitative research method?

within the past 5 years

06 March 2024 2,103 3 View

Why L. parabuchneri and L. otakiensis are not on Qualified presumption of safety list by EFSA ?

The regulatory institution European Food Science Authority (EFSA) publishes and maintains a list of organisms generally considered as safe for food production - The Qualified presumption of safety...

11 February 2024 8,511 0 View

Research on library outreach and extended services?

I am researching information on what defines outreach.

14 December 2023 4,668 1 View

When doing an analysis to select the best model based on AIC, is it normal to get a value of 1 in the AICcWt?

I am making an analysis to select the best model based on AIC, but one of my models gets a value of 1 in the AICcWt. I understand this basically means that this model has a power explanation of...

25 July 2023 550 2 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

Recruitment for Postpartum Mental Health Research?

We are currently recruiting for two studies on postpartum mental health as part of our work at the Perinatal Mental Health Research Lab at Alliant International University. If you or someone you...

30 July 2024 2,950 0 View

What is the impact of collaborations with key suppliers on an SME's competitiveness?

The literature on Supply Chain Integration (SCI), Supply Chain Management (SCM), and collaborations, spanning several decades, deserves special attention. This topic, although addressed for a long...

23 July 2024 5,672 3 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

Can anyone suggest me the questionnaire along with scoring key to assess the mental health wellbeing of the college faculty?

I am Ruthra, and I have been working as a psychologist for the past 10 years. Now, I'm interested in conducting research and I would like to conduct a survey on mental health and wellbeing among...

15 July 2024 4,119 4 View

Are diatom inclusions found in shellfish shells?

Mangroves were reported to eat into limestone atolls like so much cake - but could it have been diatom partnerships at mangrove roots that ate into the rock? If gypsum formation is a clue, how...

12 July 2024 159 2 View