Barbara Mandriani

3 Questions 5 Answers 0 Followers

Questions related from Barbara Mandriani

Where can I find a good protocol to prepare competent E.coli cells with CaCl2 in lab?

I am looking for a standardized protocol to prepare calcium chloride-competent E.coli cells. Thanks

26 February 2025 1,629 0 View

How to improve PCR for YAP amplification?

Hi, I am having trouble amplifying YAP via pcr with DNA Pol PfU. I am using PfUUltra high fidelity DNA polymerase. I would like to amplify YAP from gDNA extracted from a cell line that does not...

08 October 2024 2,385 8 View

What's the best reagent for T cell activation?

Peripheral blood mononuclear cells (PBMC) are suspended at a concentration of 1Å~106 cell per mL in T cell medium containing 50 ng/mL of the anti-CD3 monoclonal antibody OKT3 and 300 IU/mL of...

16 April 2020 6,433 0 View