Lignin-degrading fungi, such as white-rot fungi, typically prioritize the consumption of readily available nutrients (like simple sugars and other soluble carbohydrates) before turning to the more complex lignin component in a heterogeneous substrate like shredded corn-cobs. This sequential nutrient utilization strategy allows the fungi to conserve energy by using easily accessible resources first, which supports initial growth and enzyme production.

Sequence of Decomposition:

Enhancing Lignin Degradation:

To encourage faster lignin breakdown, some researchers condition substrates by partially removing simple sugars beforehand or applying mild physical/chemical pretreatments. These treatments make lignin more accessible and can promote earlier ligninolytic activity in fungi.

So, lignin degradation does not start immediately but follows the depletion of simpler nutrients. The fungi adapt by shifting enzyme production to meet the available substrate complexity.