02 February 2016 10 8K Report

I am confused regarding the analysis of qPCR data to find significance. I have n=5 biological replicates. There are 2 different school of thoughts. One thinks we should perform statistics on dct values which are normalized to endogenous gene. The other thought is we should perform statistics to fold change value where you have normalized dct values to control dct values.

IF anyone has any explanation and which method is correct with reference, i would appreciate it.

Thank you

Vrutant

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