I have seen that tomato powder can be supplemented in MRS broth (in place of tryptone), or that tomato juice can be used.
Is this just store bought tomato juice?
In addition, any suggestions for maintaining pH given the acidity of tomato juice?
Hardy Diagnostics Tomato Juice Agar is a nonselective medium for the cultivation of Lactobacilli species, especially Lactobacillus acidophilus , and other acid-loving bacteria. Tomato juice acts as a source of carbon and proteins, while peptone provides a source of nitrogen, amino acids and carbon. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/TomatoJuiceAgar.html
Check http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0113&org=82&c=UK&lang=EN
Hi,
Acidic tomato broth medium and acidic grape medium are used for isolation of wine LAB, especially a malolactic fermenting Oenococcus oeni. The organism is acidophilic and requires a specific growth factor. Tomato or grape juice provides the growth factor. Anaerobic culturing is essential for isolation of O. oeni. Antibiotics, e.g., sodium azide (10 mg/L) and cycloheximide (10 mg/L), are helpful for selective isolation of LAB.
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