What is wrong with patch clamp?

Hi, colleges, thank you for your reply. I update my descriptions in my attachment. So sorry for the miswrite of water. It is actually ACSF. I have two questions. Frist, when the pipette into the...

06 July 2014 6,529 21 View

How can I record local field potential (LFP) in free moving mice?

I want to record LFP in free moving mice but I have no idea how to do this experiment. Frist, what type of recording electrode and reference electrode to be used in recording LFP, metal or glass...

03 April 2014 3,882 9 View

the surface of slices is uneven by frozen section

These days I have some problems with the frozen section of mice brain. I used the antibody to stain the slice. Observing with a confocal microscope I found that there are a lot of black holes on...

10 November 2013 1,177 5 View

Can someone advise on an antigen retrieve experiment for frozen section?

09 October 2013 8,415 7 View

How to avoid fluorescence bleaching?

I am doing an experiment about calcium imaging in hippocampal slices. I have two questions. 1. I found that it was hard to load fluo4-am into hippocampal CA1 pyramid cells, so how to improve the...

07 August 2013 1,038 1 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

In Microstrip patch antenna ,How to turn on the diode ,which is placed in the antenna slot with DC blocking capacitor ?

In Microstrip patch antenna ,How to turn on the diode ,which is placed in the antenna slot with DC blocking capacitor ?but entire patch is in AC supply due to antenna feed..

01 February 2021 4,904 3 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View