Mao Yeh

6 Questions 17 Answers 0 Followers

Questions related from Mao Yeh

What is wrong with patch clamp?

Hi, colleges, thank you for your reply. I update my descriptions in my attachment. So sorry for the miswrite of water. It is actually ACSF. I have two questions. Frist, when the pipette into the...

07 July 2014 6,570 21 View

How can I record local field potential (LFP) in free moving mice?

I want to record LFP in free moving mice but I have no idea how to do this experiment. Frist, what type of recording electrode and reference electrode to be used in recording LFP, metal or glass...

04 April 2014 3,954 9 View

the surface of slices is uneven by frozen section

These days I have some problems with the frozen section of mice brain. I used the antibody to stain the slice. Observing with a confocal microscope I found that there are a lot of black holes on...

11 November 2013 1,219 5 View

Can someone advise on an antigen retrieve experiment for frozen section?

I want to retrieve antigen by 1% SDS at 90 ℃. In this situation, can SDS dissolve cell membrane and cause protein loss?

10 October 2013 8,487 7 View

How to avoid fluorescence bleaching?

I am doing an experiment about calcium imaging in hippocampal slices. I have two questions. 1. I found that it was hard to load fluo4-am into hippocampal CA1 pyramid cells, so how to improve the...

08 August 2013 1,080 1 View

When I saw "slices evoked spikes with a moderate jitter", I don't know what it means.

And, how to analyze the evoked data to get the parameter "jitter"?

08 August 2013 6,640 3 View