My research is looking to detect cell activation (specifically PAMs) through ROS measurement.
Dear Claudia,
CellROX oxidative stress reagents are different cell-permeable fluorescent probes that are mainly used as indicators for oxidative stress. The different CellROX probes are diffusible and cannot differ between cellular compartments and ROS subspecies, but vary greatly in usage conditions, like live cell compatibility, resistance to detergents, fixation capabilities and emitted fluorescence. CellRox Red is not resistant to detergents while CellROX Green is resistant
If you like, have a look at the pdf of the distributor:
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp10422.pdf
if you like, you can have also a look at our review:
Article Functions of ROS in Macrophages and Antimicrobial Immunity
It also shows the mistakes people make with interpreting ROS measurements, when using wrong ROS probes or ROS scavengers.
If you have any further questions please ask any time.
All the best and stay healthy,
Marc
Dear Marc,
Thank you very much. I will take a look to the shared literature.
Stay healthy too.
Claudia
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