Hi Alexis,

During the time when the cells begin to adhere to the culture plate, is when they express surface proteins. The time would vary for each cell type depending on their doubling time mainly and confluency.

Hope this helps.

Shefali Mehta , thanks you for the answer. I think that these times would need to be standardized.

They already have been for most of the cell types. You may want to search in standard books for it or observe it yourself.

Make sure the confluency is around 70%

Fine, Shefali Mehta

I will look for those informations, Thanks!!!