This nomenclature has confused me for years, and I have been told different answers. Does 1:1 25 mM AmBic/50% ACN refer to
A) a final concentration of 25 mM AmBic and 50% ACN,
B) equal amounts of 25 mM AmBic and 50% ACN mixed (final 12.5 mM AmBic and 25% ACN) or C) something else entirely?
If A, Then what do you do with recipes that call for 1:1 25 mM AmBic/ACN (implying 100% final concentration)?
Thanks for the clarification!
My interpretation is that you are meant to mix in equal volumes a solution of 25 mM AmBic and a solution of 50% ACN, with the result being 12.5 mM AmBic and 25% ACN (i.e. answer B).
In the second case, the resulting solution would be 12.5 mM AmBic and 50% ACN.
Hi Ryan,
I think that B is the correct answer. Usually Mixing solvents with statement 1:1 means equivolume mixing of components, (1+1=2volumes diluted finally x2) which finally yields halved concentrations of constituents. This should be premixed if used as Hplc mobile phase and degased, and pumped through special pH basic resistant column matrix.
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