How do you get around this AutoDock Vina error?

I get the following error messages after running the simulation: Sorry, there are no Gasteiger parameters available for atom ligandx.ligand:A:lig1:O7 AutoDock Vina ligand docking initiated for...

26 January 2021 3,822 3 View

Speaking of the Brazilian test of Lobo Carneiro, which other researchers did similar tests?

22 October 2020 4,536 2 View

Why is SDS-PAGE incorporated into Western Blot over native PAGE?

I know both can be used, but why is SDS-PAGE "preferred"? Does it have to do with cross-reactivity?

29 March 2020 6,000 5 View

What is the best etchant for 3d-transitional metals high-entropy alloys?

I would like to reveal the as-cast dendrite microstucture.

30 January 2019 9,690 3 View

Metagenome Contig Taxonomic Classification Approaches?

If you were a researcher interested in investigating a particular group of prokaryotes (e.g. Cyanobacteria) from an environmental sequencing project, which of the following approaches to taxonomy...

26 October 2018 7,027 3 View

Is there an easy way to determine the area of a raster inside a polygon/shapefile?

I have a (1) polygon/shapefile of administrative boundaries and a (2) a raster with different categories (e.g. 1, 2, 3, 4, 5,) I would like to determine the area of the raster (only category 1)...

18 October 2018 4,545 5 View

Did someone are using RNAstable with RNA samples in a elution buffer instead of DPCE -traeted water?

31 December 2017 2,045 1 View

How to get representative sample of fungi/micellia bacteria from a reactor 10L?

Hello, I am dealing with the sampling of fermentations which becomes very viscous due to micellia. We have a sampler but I suspect the tube is too thin and we are not getting a representative...

30 August 2017 2,174 3 View

Does the concentration of purified pcr product affect the result of NGS community distribution?

Although the loading mass for NGS would be the same, I am just thinking if it matters if my DNA concentrations after purification varies a lot (e.g. 20 vs 200 ng/uL)?

21 January 2017 1,798 1 View

What spider species is this?

Have a prosperous new year to everyone! What spider species is this found in a commercial swimming resort in Jasaan, Misamis Oriental, Philippines. Please help in identification

01 January 2017 5,295 10 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View