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More Bruce Robin Nyamweha's questions See All

How many tumor cells should I seed in a 96-well plate/well for virus infectivity?

The tumor cells are from a variety of cell lines from ATCC .

22 December 2020 1,950 3 View

How does band gap of a semiconductor shift with the lattice strain? and physical significance?

Someone posted a similar question but I have a further question on strain (increase/ decrease atom distance). So I know that the distance increases and decreases the bandgap distance. But can it...

04 June 2020 689 5 View

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance and yield attributing traits self pollinat?

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance in self pollinating crops (F2 generation) Vs cross pollinating crops, is it important to...

30 March 2020 1,011 3 View

What is the most appropriate statistical test for my experiment?

I am having trouble identifying the most appropriate statistical test for an experiment I am designing. In this experiment I am looking at multiple markers that indicate a change in structure...

25 November 2019 9,090 2 View

Where could I find some SOPs and formulations for making in house Clinical chemistry reagents?

Hi, I am looking for some resources to be able to find some SOPs and formulations for making in house Clinical enzymatic chemistry reagents like; Triglyceride, HDL-Cholestrol, Oxalate, Lactate,...

25 July 2019 698 2 View

Has anyone else had any problems with PSA antibodies for WB?

I am doing work on prostate cancer cell line - LNCaP - and using PSA antibody to show androgen deprivation. We currently have 3 in our lab, none of which are giving us a signal for western blots....

14 July 2019 2,723 3 View

When thawing cryopreserved cells, can they be added to 0.9% NaCl without loss of viability?

The cells are cryopreserved in 8% DMSO and 2% HSA.

08 November 2018 3,807 3 View

What is best way to check for non-proliferation in a tumor cell lysate?

We use 6x freeze-thaw cycles and consistently show 0% viability by Trypan Blue. We have used MTT and results were good, but some say DNA assays are better. Thanks

03 August 2018 4,119 3 View

What is second order CFA?

Hi ? I ran CFA in AMOS and fit indices were poor . I ran EFA in SPSS (etracted six factors) and delete some Items that were cross-loading , then run CFA again and now fit indices are within...

30 July 2018 9,679 5 View

Any antibodies against MMP2 and MMP9 that are suitable immunohisto?

Hello All. Can anyone suggest antibodies against MMP2 and MMP9 that work well for immunohisto in the mouse heart? Thanks in advance. Bruce

24 November 2016 7,284 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View