at 18 and 24 hours my cells are not showing MMP decrease as literature suggests 18 hrs. anyone having a personal experience?
I have not used JC1, but I have used TMRE to measure mito membrane potential by flowcytometer. I would suggest you to use serum free media while treatment and check once; also do use CCCP/FCCP (50-100uM) for 10mins as a control.
Thanks govardhan!! Ll try today!
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