I am planing to test Treg cells proliferation after activated by TLR2 agonists. I have read some papers and found that some researcher use [3H]thymidine incorporation as a readout, while in the same paper they also use CFSE as a way to test cell division. i don't know the difference between these two measures, can anybody help me ?thanks very much .
Both could be used to study Treg cells proliferation. CFSE could be measured using flow cytometry and [3H]thymidine as radioactive compound detected differently by radioactivity ( you need special equipment to measure it). Both works fine and I recommend CFSE staining as it simple and it works fine. Good luck.
tritium is radioactive, and although not very dangerous it needs some safety considerations. CFSE is nice and crisp on flowcytometry, in my current lab we use Cell Trace Violet which is giving good results similar to CFSE. As Fadi mentioned, CFSE as well as the cell trace dyes are simple to use and can be added to any flow panel for further analysis giving you the option to look at phenotype and proliferation at the same time without losing your precious samples. Good luck.
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