In case you are still following this link: All the above mentioned procedures are suitable to obtain conidia suspensions. However, regardless the method you are using, you should filter the resulting conidia suspension over a 40 µm cell-strainer. Although this will result in loss of a substantial proportion of your conidia, this is the only method to get rid of hyphal fragments, conidiophores and larger clumps of conidia. With several Aspergillus species it helps to add 0.05% Tween while harvesting your conidia, because it reduces the number of clumps. However, in all cases you should wash your conidia after filtering and store them in water or PBS at 4°C (for prolonged storage perform a cryopreservation). Washing is required to get rid of nutrients from your initial growth medium and prevents conidia to start swelling and, therefore, keeps viability high. However, after prolonged storage in suspension (>7 days) you should you should check the viability of your conidia, which should always be above 90% for subsequent experiments.

You should use the single-spore culture. First, in sterile conditions lead acsenic strain. Then, using a needle or thin injection needle take one spore from culture - microscope is needed, of course, on which manipulations can be carried out on the culture. Then move the spore to the new substrate. If you keep sterile conditions, a culture that has grown after incubation is pure. Spores from single-spore culture You may take with needle (then they will be single) or carefully with loop, you can then transfer it to a sterile saline and make suspension with the required density. All in sterile conditions, laminar chamber for example.

Wish You best luck with experiment.

prepare a slant of Water Agar (2% agar and 100 ml tap water) in a 250 ml Erlenmeyer flask. innoculate 10 mm mycelial disc of the aspergillus and incubate it at 28C for 10 days. After sporulation wash it with physiological saline (say 10-20 ml), The spores will be harvested and the color of the saline will become black. You may further use it for your experimental works. All the work should be carried out in sterile conditions. All the best for your experiemnts

Grow Aspergillus in Cellophane paper laid over PDA media. Harvest the mycelium and use tween 20 violently agitate the mycelium spores settle at the bottom.

In case you are still following this link: All the above mentioned procedures are suitable to obtain conidia suspensions. However, regardless the method you are using, you should filter the resulting conidia suspension over a 40 µm cell-strainer. Although this will result in loss of a substantial proportion of your conidia, this is the only method to get rid of hyphal fragments, conidiophores and larger clumps of conidia. With several Aspergillus species it helps to add 0.05% Tween while harvesting your conidia, because it reduces the number of clumps. However, in all cases you should wash your conidia after filtering and store them in water or PBS at 4°C (for prolonged storage perform a cryopreservation). Washing is required to get rid of nutrients from your initial growth medium and prevents conidia to start swelling and, therefore, keeps viability high. However, after prolonged storage in suspension (>7 days) you should you should check the viability of your conidia, which should always be above 90% for subsequent experiments.