How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

I am looking for some suggestions for my PhD research topic in Management Science and Engineering, pls suggest me some new and innovative topic?

Please suggest me some topics.

26 December 2020 3,762 16 View

How to calculate Basis Set Superposition Error (BSSE) for interaction energy in SIESTA 4.0.1 code?

Hello, I'm calculating the interaction energy between molecule and graphene sheet using Eq. (E_i = (E_graphene + E_molecule) - (E_graphene+molecule). I have employed a numerical atomic orbital...

10 November 2020 8,467 2 View

How can I ensure that finite strain formulation has been used in the ABAQUS simulation?

I want to simulate a problem in ABAQUS through two different theories 1. Finite strain theory and 2. Large-deformation or large-rotation theory with small strain assumption. Since both the...

05 November 2020 1,634 1 View

How we can keep same amount of DMSO in all serial dilutions when we are using "2-fold serial drug dilution method" ?

How we can keep same amount of DMSO in all serial dilutions when we are using "2-fold serial drug dilution method" by transferring double amount in first test tube or microtiter well then taking...

10 January 2020 1,822 4 View

Can i define composite section using section designer tool of sap2000?

I wanted to carry out retrofitting by applying steel plates to the concrete section. Can it be done by sap2000 section designer tool?

11 November 2019 2,252 2 View

How can i import the results from running MATLAB script in Crystal ball application in order to perform Monte Carlo analysis for the same?

I want to perform a Monte Carlo simulation in Crystal Ball application, by running the inputs in a matlab script and getting outputs from it to be pasted in forecast analysis cells in crystal ball.

07 July 2018 2,856 3 View

What are the best bioinformatics tools for analyzing the effect of missense mutations in plants?

I want to study the effect of amino acid substitution in a flowering related protien gene in soybean, please suggest me available bioinformatics tools?

21 May 2018 5,696 3 View

Why do some compositions form eutectic?

I want to know about the reason leading to a particular composition of a metal mixture to form a eutectic alloy. I know that Gibbs free energy plays a very role and I can figure out the...

29 December 2017 4,328 5 View

Can someone help me with Pb-Bi-Sn ternay phase diagram at room temperature?

I am in need of ternary phase diagram of Pb - Bi - Sn alloy system at room temperature. I would be so grateful if someone could help me obtain it.

29 November 2017 1,450 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View