Hi, I have observed abnormal 2-cell embryo lysing in both the experiment group (electroporation) and the control group ( untreated).
Embryos were collected at 21.5 hours post-hCG. Electroporation was done at 24.5 hours post-hCG. Electroporated embryos and untreated control were cultured overnight in a CO2 incubator.
The timeline and data of one of the culture dish are listed here:
Embryos were graded at 43.75 hours post-hCG (7:45 am):
#2-cell: 12
#lysed: 26
#1-cell: 1
#fragmented: 5
8:06 am observed morphologically normal 2-cell
8:08 am observed the morphological change in 1 embryo: both cells were enlarging
8:09 am observed more changes in this embryo: both cells were fusing together
8:16 am observed more changes in this embryo: 2-cell morphology disappeared. Observed one-cell like shape. Pale.
I wonder what would possibly explain the 2-cell embryos morphological changes in 10 minutes. Thank you for your help!
Yang
Vitrification of mouse embryos at 2-cell, 4-cell and 8-cell ...
https://www.ncbi.nlm.nih.gov › pmc › articles › PMC2799565
by J Zhang