Yang Liu

3 Questions 4 Answers 0 Followers

Questions related from Yang Liu

%FBS in hybridoma cell culture?

Hi, most people use SFM to culture hybridoma cells, but some use 10% FBS. Is using SFM or 2% FBS/DMEM for hybridoma cells a golden standard?

11 June 2020 3,398 0 View

What would cause mouse 2-cell embryo lyse?

Hi, I have observed abnormal 2-cell embryo lysing in both the experiment group (electroporation) and the control group ( untreated). Embryos were collected at 21.5 hours post-hCG. Electroporation...

11 November 2019 5,841 1 View

Any one has problem with random donor insertion when making KI mice using Crispr/Cas9?

Is the chance that donor vector was integrated into genome randomly high or rare?

25 May 2016 7,502 1 View