I tried TUNEL assay to know the apoptosis rate in cryopreserved bovine embryos using APO-BrdU™ TUNEL Assay Kit (Catalog Number A23210)but not getting expected result so what would be the probable reason for these ????
Did you use positive control slide, If yes..did you get TUNEL positive staining? If no....recheck your kit materials and protocol to get expected results.