Mehak Kaushal

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Questions related from Mehak Kaushal

Trying to amplify a gene from genomic DNA with primers with low GC content and attached restriction sites, what would be the optimum PCR conditions?

Forward – 5’GAC GGA TCC ACG CAT TAA AGT ATA TTG TGT 3’ (Tm- 66.2 °C) 30bp (with Bamh1 site) Reverse - 5’ GCC TCT AGA AAT TAA TGA GCG CTT TAA ACT 3’ (Tm- 65.2 °C) 30 bp (with Xba1 site) My gene...

04 April 2014 2,938 6 View

What would be the key difficulties found in expressing a membrane protein from gram negative bacteria in gram positive bacteria?

What would be the key points to be addressed while expressing a membrane protein from gram negative(E.coli) in gram positive clostridium acetobutylicum?

02 February 2014 2,063 0 View