I’m working on mouse cardiomyocytes and I wonder if different energy providing substrates would perform differently in cardiomyocytes in matter of force development, excitability, viability. I tried to image ATP by indirect measuring with Magnesium-Green, but could only find effects due to Na-Azide, but not due to feeding different substrates. Next I tried to take spontaneous calcium signaling as measure but the gained results were rather inconsistent. Now I wonder if different energy substrates would have on effect on force development and/or heart performance. Please let me know if you have made some experience or if have a better idea.
Hi Izabela, thanks for your answer. Maybe oxygenintake would be interesting for me aswell. I'm mainly interested in the performance of lactate on the heart metabolism. Some groups reported that cardiomyocytes consume lactate, especially during strong physical activity. I studied, so far, lactate transport kinetics and could alter those by keeping the cells in different conditions. Now, this is quite nice but useless if those altered transport kinetic do not result in altered heart performance.
Hi Jan, we apply in vitro myocarial mitochondrial ATP-content and production analysis by chemiluminescence combined with mitochondrial respiratory chain analysis using a clark type elctrode and analysis for mitochondrial membrane potential determination by JC1. In our model of global myocardial ischemia induced in a Langendorff apparatus we achieve reliable data. Contact me if you need detailed information. With best regards
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