What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

Fluorophore panel selection?

Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.

19 January 2021 3,475 3 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

How to standardize and set parameters in the Puller sutter P-97 to get accepted pipette shape and resistance for whole cell recordings on DRG?

We are working on a P-97 puller and we are using it to do whole cell recording in DRG. We have 2 types of pipettes (borosilicate glass ID: 1.1 OD: 1.5 and ID: 0.86 OD: 1.5 ) as it showed in the...

04 January 2021 2,112 6 View

How to solve carry-over and interference problem when detecting tulathromycin by LCMS?

I am working on the determination of tulathromycin in rat faeces using QE-focus (Thermo Fisher)(Waters BEH C18, 2.1x100mm, 1.7um)(0.1%FA+H2O, 0.1%FA+ACN) . I encountered two tricky problems....

02 January 2021 2,713 5 View

Why dose plasma AngII concentration decrease in AngII infusion mice detected by AngII ELISA kit?

I detect the plasma AngII concentration by AngII ELISA kit (ENZO), the results show that plasma AngII decrease in AngII infusion mice. I check with the kit and find that the kit could recognize...

31 December 2020 8,838 3 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

Why isoelectric point (IEP) of alumina (Al2O3) dispersion decreases with the increasing of calcination temperatures?

We have prepared different alumina powders (fine/submicron-sized) from waste sources at different calcination temperatures (C.T.). From zeta potential-pH graph, we saw that IEP values decreased...

09 January 2021 9,404 3 View

Can we mix elemental powders of 325 mesh using an agate mortar and pestle?

Elemental Powders like Titanium, vanadium, tungsten, zirconium, hafnium.

04 January 2021 7,357 1 View

How to get nanocomposite of PEEK with Zirconia ?

Need a lengthy PEEK with Zirconia based nano composite of 180 mm length. Which method is possible? Tried injection molding no proper mix. Solvent casting process is also very costly. Kindly anyone...

13 December 2020 4,329 5 View

What is the difference between calcination and pyrolysis.?

please tell me in case of carbon material of metal oxide

09 November 2020 5,572 5 View

What are the XRD analysis conditions of the cement, sand and Limestone ?

I am a Phd student and I need to do XRD analysis on my pouder samples namely ( cement- sand- limestone and calcined clay). I was asked asked to specify the analysis conditions: -the nature of...

07 October 2020 5,737 6 View

What is the driving force for the Stress Induced Transformation Toughening (one of the toughening mechanisms in ZIRCONIA TOUGHENED ALUMINA)?

It has been established that the tetragonal to monoclinic phase transformation of zirconia can be used to improve the toughness. The driving force for this transformation is the temperature...

01 October 2020 9,634 4 View

How to avoid getting organic particles to charr(become black) during calcination?

I am trying to synthesize SrHfO3:Eu2+ nanoparticles. To reduce from Eu3+ to Eu2+, we calcinate it under Ar/H2 conditions but it ends giving a black coloured product. We need this for luminescence...

22 August 2020 448 3 View

Synthesis of black TiO2 powder by vacuum calcination?

I have synthesized TiO2 powder through sol gel method. I tried to do calcination of this white TiO2 powder under vacuum at 500 deg C. No change in colour was observed. Is this normal? because I...

20 August 2020 5,425 4 View

Will e.max survive the battle with evolving zirconia ceramics?

Zirconia appeared in the dental market as a strong yet ugly ceramics. They were Fit only as frameworks that to be veneered later by more esthetic feldspathic ceramics. It was indicated only in...

01 August 2020 2,865 5 View