Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Database of European Football Players Salaries ?

Hello everyone, I am interested in writing about a dissertation about the wages of the football players of the 5 main leagues in Europe. I wanted to know if someone had an up to date data base...

17 February 2021 8,847 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

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01 January 2021 5,831 1 View

Are there only winners in the world of exism?

BREXISM finally is over and they got a trade deal with europe now, but exism left the UK poorer according to the news Brexit is finally done. It will leave the UK...

23 December 2020 4,071 6 View

New Covid virus came to Europe, Australia then spread other part of the world, do you think that previous vaccine will effective for recent virus?

New Covid virus came to Europe, Australia then will spread other part of the world, do you think that previous vaccine will effective for recent virus?

21 December 2020 4,256 15 View

How much of the bird trade does CITES cover?

Trade in wild-caught birds is big business. Most data on which species are traded and in what volumes comes from CITES. But the CITES Appendices do not cover all traded bird species, e.g. it does...

30 November 2020 1,955 4 View

IHC marker for cytoplasmic staining of oligodendrocytes?

Hi, I'm planning on doing oligodendrocyte staining on frozen and paraffin brain sections of postnatal (up to 2 weeks old) and adult mice. I was wondering which antibody would you recommend...

18 November 2020 8,871 2 View

How to increase presynaptic neurotransmitter release artificially?

I would like to pharmacologically increase presynaptic neurotransmitter release in mice CNS in vivo (through the extracellular micro-infusion of a compound). Which strategy would you recommand?...

02 November 2020 5,645 3 View

Do you know any examples of ethnic stereotypes reflected in literature on wooden architecture in Central and Eastern Europe?

In 19th and 20th centuries wooden architecture became very popular among researchers and enthusiasts of vernacular culture in Central and Eastern Europe. Numerous texsts by professional scholars...

18 October 2020 9,659 2 View

How do you think why fiber-optic DAS systems are not frequently used in bioacoustics?

There are some interesting challenges for DAS systems in fields of agri-biophotonics and/or biophotonics - from vibration impact studies on the roots of growing plants to sea fauna acoustics...

16 October 2020 6,696 5 View