What w/v% of H2O2 should I use to bleach cotton fabric - with severe moulds colonization - using cold water?

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Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Use magnetic bead purification after gel electrophoresis?

Hi, I am working with Invitrogen Seamless cloning which requires 200 ng/ul insert DNA fragment. Protocol recommends to use restriction enzyme to digest pre-cloned plasmid and then elute specific...

25 June 2024 971 2 View

How to set up focus groups in the face of generational barriers?

I am carrying out a research project in which I am conducting focus groups with older men and women to study the musical stereotypes learned in traditional Spanish oral music groups, but I am...

10 May 2024 3,852 3 View

What is the best LC/MS system for surfactant analysis?

We are an R&D laboratory focused on anionic, cationic, and zwitterionic surfactants and usually tackle surfactant synthesis and analysis of products from customers. In particular, we deal with...

29 April 2024 8,927 1 View

Why is there no 28S RNA in mouse sceletal muscle?

We are isolating total RNA from mouse sceletal muscle, using the beads beater with the sample already being in Trizol, afterwards chloroform extraction and finally Qiagen RNeasy kit. On the TAPE...

29 April 2024 3,952 5 View

What’s the most used inclusive leadership assessment scale?

I’m looking for an inclusive leadership scale to include in a mediation analysis with psych safety and workplace belonging, what would you use

27 April 2024 6,072 2 View

COMET ASSAY: Why do cells disappear?

Greetings, I am trying to set up an alkaline Comet Assay. I have no previous experience with this type of assay, unfortunately, so I am doing a lot of trial and error. After embedding my cells in...

23 April 2024 6,534 0 View

In what ways does the Sysmex DI-60 streamline laboratory workflow and improve laboratory efficiency in terms of reduced TAT?

This question is directed on the performance of the Sysmex DI-60 digital morphology analyzer and its contributions on the improvement in the laboratory workflow. I am curious as to what...

10 April 2024 3,069 1 View

Aside from a lack of understanding, what other variables contribute to antibiotic diversion in low-income countries such as the Philippines?

It is known that self-medication with antibiotics is an arising problem, becoming a major constituent to antibiotic resistance. Moreover, self-medication heightens the possibility of developing...

09 April 2024 6,644 1 View

What are the most prevalent antibiotic resistance patterns among outpatient populations in the Philippines?

Antibiotic resistance is a pressing global and local health concern. Outpatient settings play a crucial role in antibiotic consumption and the emergence of resistant bacteria due to the frequent...

09 April 2024 6,004 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Is it possible to improve the reforming section of the Haber-Bosch process without using air separation units?

A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...

01 August 2024 7,051 5 View

How can the rubber fibres produced by the electrospinning device be removed from the fibre collect(aluminium foil) ?

How can the rubber fibres produced by the electrospinning device be removed from the fibre collector (aluminium foil) without affecting the orientation of the rubber fibres for use in reinforcing...

26 July 2024 8,281 0 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

Appearance of crystallization peak in heating cycle of DSC test?

For which types of polymers, the crystallization peak in heating cycle of DSC test can be observed and for which types cant? In another word, when this exothermic crystallization can be observed...

18 July 2024 348 3 View

How do you decide an optimal AC amplitude for non-faradaic electrochemical impedance spectroscopy?

I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...

16 July 2024 5,031 1 View

Would anyone care to recommend an inexpensive waterproof serviceable (e.g., batteries can be changed) temperature logger?

I've been using Hobo pendants for years but they are up from less than $50 a few years ago to over $100, and I'm in need of an alternative.

08 July 2024 7,164 1 View

Is it possible to define exothermic processes in finite element software like COMSOL? If yes, how can this be done?

We aim to study an exothermic process and evaluate its temporal changes. Could you recommend suitable software for this purpose and explain how to conduct this analysis? What specific data is...

30 June 2024 8,413 1 View