If an observed d falls beyond the upper limit in equivalence testing, what does that mean?

I have been taking your Coursera Classes and reading some of your articles on equivalence testing, and I do not know how to interpret and effect and its corresponding CI, but I do not how to...

12 October 2020 9,046 5 View

Hi, does anybody have a good (without cracks in brain sections) Nissl staining protocol in Zebrafish or any other fish?

Hello everyone, I have stained brain sections of Zebrafish using Nissl staining and I watched cracks in the tissue :B . It's quite disturbing. Any help will be appreciated. Thanks :)

21 May 2018 1,907 8 View

Hi, does anybody have a good (without cracks in brain sections) Nissl staining protocol in Zebrafish or any other fish?

Hello everyone, I have stained brain sections of Zebrafish using Nissl staining and I watched cracks in the tissue :B . It's quite disturbing. Any help will be appreciated. Thanks :)

04 May 2018 3,963 9 View

Separate high molecular weight proteins performing a SDS-PAGE

Hi everyone! In a SDS-PAGE, what's the more effective way to separate high molecular weight proteinst (>100 kda) and get a good resoluion at the end? I know that the polyacrilamide gel...

13 May 2017 3,349 4 View

Please send me your article

Review Article Epidemiology of Neuromyelitis Optica in the World: A Systematic Review and Meta-AnalysisMasoud Etemadifar, Zahra Nas

04 May 2015 9,888 1 View

Does anyone here knows how to perform a post-hoc test?

I desperately need some help regarding with my statistical analysis prior to my undergraduate research study.. What are the best post hoc test that can be perform for pairing two groups with...

03 October 2012 7,416 22 View

Thomas Jefferson experiment

"No experiment can be more interesting than that we are no trying, which we trust will end in establishing the fact that man may be governed by reason and truth. the most effectual hitherto found...

11 February 2012 5,937 1 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View