03 November 2016 1 8K Report

i am doing research on service recovery quality of shopping customers in select cities in India. i need statistical tools and latest techniques of service recovery. can you give me the advise of my question. thank you

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How to design a primer?

Hello, If the gene our interest to amplify does not follow any criteria in designing primers how to proceed with that sequence? If the gene sequence is1000 - 1500 bp. Thank you, Ramanjaneyulu

14 May 2020 1,647 15 View

How to clone ?

Hello, If I want to clone a gene of interest in a plasmid containing ORF, how should I do that, and which criteria I should follow? Thanks, Ramanjaneyulu

14 May 2020 9,617 2 View

How to calculate saccharification %?

Hello Every one, I have done saccharification with 50 U/ml crude enzyme extract in 50 ml buffer with 5% solid loading. Now I have a doubt at calculation part. If I get released reducing sugar of...

20 March 2016 9,739 10 View

How can one become able to select particular concentration of enzyme in U/ml?

Hello Every one, I am working on saccharification of biomass with crude enzyme. In this case I need to select  certain concentration of enzyme (eg: 500 U/ ml), If suppose my enzyme...

18 November 2015 6,000 2 View

What is a bootstrap value?

What is bootstrap value? what does it denotes? Is it necessary for the construction of phylogenetic tree and what is the best no. of bootstrap replicates value for construction of fungal...

12 July 2015 2,461 16 View

Which is the best on-line database for the construction of a phylogenetic tree for fungi?

Hello Every one, I have isolated and sequenced ITS regions for identification of  fungi by Sangers method. To construct a phylogenetic tree  which is the best tool available in on-line...

08 March 2015 9,594 13 View

How can I optimize culture conditions of enzyme production by using Response Surface Methodology?

Hello Every one, I have done experiments on enzyme production and optimization of production parameters like temp, pH,  ...etc. Now I have to optimize the significant parameters by using Response...

06 January 2015 6,331 4 View

What is the best method to isolate/extract fungal genomic DNA?

What is the best method to isolate/extract fungal genomic DNA? I have tried several methods without the addition of chitinase, but I wasn't successful in this aspect. Please suggest a better...

10 December 2014 7,606 9 View

What is the role of acids in lignocellulosic biomass pretreatment?

Hi all,May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?If we...

12 October 2014 5,982 10 View

Is there any method for detection of Glu, Xylose and Xylitol by HPLC?

Hello Everyone, Please let me know, if there is any single or different methods available for detection of glucose, xylose and xylitol by HPLC equipped with C18 column and UV detector. If not...

19 August 2014 8,881 14 View

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