How to design a primer?

Hello, If the gene our interest to amplify does not follow any criteria in designing primers how to proceed with that sequence? If the gene sequence is1000 - 1500 bp. Thank you, Ramanjaneyulu

14 May 2020 1,417 15 View

How to clone ?

Hello, If I want to clone a gene of interest in a plasmid containing ORF, how should I do that, and which criteria I should follow? Thanks, Ramanjaneyulu

14 May 2020 9,455 2 View

Which is the best on-line database for the construction of a phylogenetic tree for fungi?

08 March 2015 9,444 13 View

How can I optimize culture conditions of enzyme production by using Response Surface Methodology?

06 January 2015 6,209 4 View

What is the best method to isolate/extract fungal genomic DNA?

10 December 2014 7,479 9 View

What is the role of acids in lignocellulosic biomass pretreatment?

Hi all,May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?If we...

12 October 2014 5,833 10 View

Is there any method for detection of Glu, Xylose and Xylitol by HPLC?

Hello Everyone, Please let me know, if there is any single or different methods available for detection of glucose, xylose and xylitol by HPLC equipped with C18 column and UV detector. If not...

19 August 2014 8,733 14 View

How does one quantify Cellulose, Hemicellulose and Lignin?

17 August 2014 2,861 22 View

How to find the synthesized derivatives of natural compounds which are synthesized earlier or not?

We are planning to synthesize the new derivatives of natural compound (Ex-curcumin) has not yet synthesized elsewhere. Hence, please let me know the tools to find out all derivatives available for...

17 July 2014 4,859 3 View

Does anyone have experience with biomass (Lignocellulose) pre-treatment?

I am working on bioethanol production. How can I quantify the reducing sugars percentage (glucose, xylose, arabinose,galactose, etc.) by using HPLC, after pretreatment, and what are the column...

07 July 2013 9,178 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View