I want to do a thin layer chromatography of an aqueous extract of plant, I used a combination of ethyl acetate and cycliheximide, no proportion gave a separation of the compounds of the extract. I tried different combinations of water and methanol, no more separation. what other solvents could you suggest to me?
What are you looking for and what are its/their properties? Did you remove the water before chomatography?
I am looking for active molecules, could be secondary metabolites like flavonoides , terpenes...
yes i remove water by evaporation.
What are your secondary solvents?
I will not solve your problem but your answers might help others
You can fragment the plant extracts using a series of different solvents in the polar (chloroform, diethyl ether and water)
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts