Membrane protein missing from SDS-PAGE gel?

I am trying to run an SDS-PAGE after his-tag purification of a sample. The protein of interest is an oligomerising membrane protein with an YFP tag attached. When the fractions of the sample from...

07 September 2020 3,277 6 View

Tool to calculate lag between BOLD time series?

Dear community I would like to ask if you are aware of any tool (Matlab/toolbox, etc.) that calculates the "lag between BOLD time series", as described in the following publications:...

01 November 2018 2,428 2 View

Is it necessary to make students prepare class reports as well as insect collections?

Dear All, I need the experience, data and opinion of zoologists/entomologist teaching zoology/entomology for BSc or MSc courses. It is necessary to make students prepare reports on the...

18 May 2017 7,405 15 View

T47D and A2780 growth problem.Why?

We work for many years with different gynecological cancer cell lines with the usual conditions. Two of these are T47D (breast) and A2780 (ovarium). For a month, this two cell lines are growing...

26 March 2017 5,358 7 View

Is there any side effects of eating rice or grains with read meat?

Red meat is harder to digest than for example poultry. If you eat a high carb meal with red meat the higher ptyalin production can increase the pH in the stomach resulting in bad digestion. Is...

03 March 2017 2,602 10 View

In what deeper sense can you speak about the quasiclassicality of the DNA macromolecule?

You can see under an ordinary light microscope that a DNA polymer is nearly a macroscopic object. But in a strict quantal sense can we expect quasiclassical behaviour, i.e., describability by a...

03 February 2017 7,734 2 View

Does anyone know about a Gene Expression Library (RNA-Seq), containing gene expression values across different cell-types and cell lines?

The ones I could find so far are highly biased towards cancer, and I needed a representative library. Does anyone know about such resource? Thanks

09 January 2017 122 3 View

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm. Can anyone help?

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm in my doctoral thesis. I would acknowledge and reference any provided SECM data. The...

30 May 2016 1,666 2 View

No title

01 March 2016 5,806 6 View

In MTT assay a cancer cell line growth activity (untreated control cells) is decreased in repeated measurements. Why?

We usually use MTT assay with more cervix carcinoma cell lines to detect antiproliferative effect of drugs. At the end of these measurements we get absorbance values according to produced formazan...

13 October 2015 9,429 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

If you haven't computer? how you show the photons to pupils?

Educational Process

01 March 2021 1,827 1 View