Hello all,
the title really says it all.
I am analysing T-cells in the context of immune dysregulation and I have developed 5 panels, each with around 10 colors (some, but not all (obviously) shared across panels).
I am struggling to see how I could use some of the tools that help deal with high-dimensional data to analyse my work. PhenoGraph, FlowSOM, viSNE and all those tools are helpful if you have all the "colours" in one panel, such as in mass-cytometry, but less so when they are spread out between different tubes.
What would you recommend?
Thanks for any ideas!
Adam