What to do when cells are not getting frozen in -80C in cryocooler?

26 August 2023 0 878 Report

I have made some freeze downs of cell lines and then kept in cryocooler at -80C for 24 hours , still after 24 hours cells are not frozen(liquid phase)

Badges
Science topic

More Chetna Yadav's questions See All

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How to select target material for X-Ray generation?

Generally, the target material is Mo, Co, Cu, Fe, Cr, etc, so on what basis should we choose this material?

31 July 2024 5,824 0 View

How to use differential equations to apply hybrid production in an inventory model ?

give suggestions

22 July 2024 5,664 2 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

Could someone please help me to understand the role of the quarter-wavelength principle in optimizing the thickness of perovskite solar cells?

How does the quarter wavelength affect the efficiency and light absorption of the cells, and what considerations need to be taken into account when applying this concept to optimize the thickness...

10 July 2024 647 5 View

How does the crystallite size and microstrain distribution influence the mechanical properties of a ceramics?

10 July 2024 3,444 4 View

What are the trending topic to writ e a review article on it?

The topic should relate to pharmacy ,drug, methos of drug discovery, or any other topic but related to dug or pharmaceutical field

02 July 2024 4,031 3 View

List Plasmid suitable for Homology cassette in CRISPR-Cas9 protocol for Knock-In in mammalian cell line ?

Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...

25 June 2024 1,197 0 View

How precisely one can do impedence or diielectric studies of high Curie temperature material (i.e., KNN based materials) with silver electroding?

Will silver coating be able to hold such a high temperature? And will there be any effect of coating thickness?

23 June 2024 8,550 0 View

Why National Human Rights Commission Is Not Fully Capable To Check The Violation Of Human Rihgts In India?

1. Role of NHRC in India. 2. UDHR 3. Constitutional rights

19 June 2024 9,616 3 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How can we conduct DFT for solutions or mixtures ?

Can we conduct DFT studies for Ionic Liquids with Amines at various concentrations? If so, how can we proceed with these studies? Additionally, what parameters, besides IR, NMR, and HOMO-LUMO, can...

18 July 2024 319 1 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

Can a liquid metal-ligand sample be directly analyze in flame atomic absorption spectrosocopy?

Can I directly analyze a liquid metal-ligand sample without acid digestion in flame atomic absorption spectroscopy?

25 June 2024 1,866 2 View

How to size an liquid jet ejector to raise pressure of suction gas?

How to size an liquid jet ejector to raise pressure of suction gas.I need to get dimensions for simple compression and also for absorption of say CO2 in Water

13 June 2024 4,551 0 View

Comment les organismes d’insectes sont-ils convertis en élément liquide ? Il pourra être étudié ultérieurement grâce à la technique GCMS ?

Comment les organismes d’insectes sont-ils convertis en élément liquide ? Il pourra être étudié ultérieurement grâce à la technique GCMS

08 June 2024 5,772 0 View

Which dispersion correction method should be used with MO6-L/6-311+G for the optimization of Ionic liquid?

since dispersion correction GD3BJ is incompatible with minnesota, which dispersion correction should be added in calculation.

05 June 2024 8,004 1 View