How to evaluate motivation in scholars, teachers and students who work from home during pandemic?

Dear all, What tool can be used to measure motivation in scholars, teachers and students who are in home isolation or work remotely? Any specific tool suitable for all people involved in Academia...

08 December 2020 7,968 10 View

How to replace the liquid blocker?

Dear colleagues, In my immunofluorescence stainigs I use liquid blocker pen, before solution with 0.3% Triton X-100. This helps to avoid dripping of solutions with primary antibodies and Triton...

30 September 2020 9,134 4 View

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06 July 2020 4,133 2 View

How long can one store primers?

Dear colleagues, one year ago we put our liquid primers in a high concentration into a -80 fridge. Can we still use them for the qPCR?

11 May 2020 4,521 16 View

What is an acceptable small sample size to carry out principal component analysis for a rating scale with 16 items???

Hi Can someone tell me what would be an acceptable sample size for principal component analysis for a rating scale with 16 items. Thanks!

30 March 2020 9,756 3 View

How to insert image in introductory text of survey in Opinio?

Hi I need some technical help. I am trying to insert an image in the introduction of a survey in Opinio. I click on 'insert image' button, then I am prompted to paste the url of the desired image,...

28 March 2020 1,543 3 View

How does the Excel work with GRG algorithm?

I'm struggling with my qualification work. Excel methods solve the math problem easy, but I don't catch the idea how he does it. I need to understand it to create the code or just to describe the...

12 March 2020 6,567 6 View

Has anyone optimized ViromerRED in THP-1, H9, and MCF7 cell lines?

I am currently attempting to optimize ViromerRED transfection in THP-1, H9, and MCF7 cells. Our initial transfections with the first batch was promising, but the new viromer we received doesn't...

15 October 2019 5,053 4 View

Can anybody explain why does the line on gel disappear after 15 cycles of PCR?

We make test PCR to choose optimal quantity of cycles and amount of cDNA for amplification, and there are lines on 9 cycles and 12 cycles (same sample), but the line on 15 cycles disappear (also...

02 April 2019 3,158 7 View

Where can I get Desert Survival Problem (computer-based) for conducting an experiment?

Hello to everyone. I am very new to computer-based experiments. Currently I am looking for Desert Survival Problem software or website that can be used for conducting a lab experiment. I saw in...

22 December 2018 6,408 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

How to formulate Energy minimization in Molecular dynamics simulation for alloys using meam potential?

during MD simulation, the atoms are not moving as per required. The problem (as per my observation) lies in energy minimization technique. upon application of tensile strain in x or y direction,...

22 February 2021 380 5 View

Magnetismic Gravity in the universe?

MAGNETISMIC GRAVITY What is magnetism? Something magnetic has a + and - charge. If 2 + or - are pushed if + and - are pulled. But what is the main reason for this? Why does magnetism have a pull...

21 February 2021 9,538 3 View

Has anyone succeeded in capturing uranium atoms in a fullerene structure?

Additional questions are: What are the encapsulating atoms? How difficult was the process and what rates were achieved? What factors most seriously affect the economics?

20 February 2021 3,134 5 View

How to include additional force field in GROMACS?

I have a cellulose model system that is best described by the GLYCAM06 force field according to the literature. However, the set of force fields included in the GROMACS package doesn't include the...

20 February 2021 3,309 3 View