How to BLAST thousands of microbiome sequences?

I'm an undergraduate student completing a capstone project working to characterize a plant microbiome. I have a couple thousand fungal ITS sequences in a fasta file. Is there any way to analyze...

03 November 2019 6,622 3 View

How to get a physiological pH when making 16% Paraformaldehyde?

I made 16% paraformaldehyde from scratch (dissolving powder in ddH20). While I was attempting to pH it, it would rapidly switch from alkaline to acidic according to the pH strips we use (range pH...

08 April 2019 6,179 3 View

How can I find out which proteins possess a specific domain or motif?

10 December 2018 8,739 2 View

What are the atomic parts of learning a character based language?

I am interested in making a suite of Unity Engine video games for second language acquisition in character based languages. The language of peak interest is Mandarin Chinese. Currently, I'm aware...

29 May 2018 6,880 1 View

Is it possible to thaw an OCT-embedded brain in order to drop-fix in PFA?

27 November 2016 9,411 1 View

Are there psychometric test (i.e. PAI, MMPI-2 RF) that have been "normed" for on-duty police officers for determing mental conditions post trauma?

02 October 2016 522 3 View

To detect Granzyme B intracellular, why the cells needed to be stimulated before staining?

if the detected cellss were stimulated in vivo, the cellls in vitro still needed to be stimulated by specific cytokines? if needed, how long is suitable for stimulating?

20 April 2016 3,502 3 View

Has anyone noticed poor agreement between replicates using the qiagen one-step RT-PCR kit?

I have been using the kit above for some time now, which performs cDNA conversion and normal qPCR in the same tube. I am noticing that despite using recently calibrated pipettes and preparing my...

30 April 2015 5,024 10 View

Why does my transformation not yield colonies?

21 April 2015 4,110 8 View

What is the best method of gDNA extraction of bacteria in semen samples?

I am trying to perform gDNA extractions on semen samples targeting Mycoplasma. I am currently using the Biosprint 96 One-For-All Vet kit however am getting very poor limit of detection in spiked...

16 March 2015 162 6 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View