Several soybean cultivars were mutagenized to produce many lines of M0, M1, M2 etc. In order to determine the genetic basis of an observed phenotype, an M0 level mutant was hybridized with its original parental cultivar to generate a mapping population. The mutation responsible for the observed phenotype has now been determined. Among the many mutants some share a similar trait features as the mutant used in the mapping experiment. What systematic experiment can be employed to identify other mutants which share a common ancestry with the mutant used in the mapping experiment?
Hi,
I am not completely certain I understood your question correctly. When you say similar traits do you mean phenotypic ones? A similarity in phenotype does not mean that their genotypes are the same. Also what kind of mutagenesis was used? Was it chemical or some method to specifically target a gene or region of the genome. In chemical mutagenesis practically every M0 seed will give rise to a different mutant line with distinct mutation or set of mutations. I am assuming it is chemical if you need to map it. In that case you can simply look for the individuals that are phenotypically similar and map them to determine the mutation. What do you mean by common ancestry?
Thank you@Svetland！The mutants were generated via chemical mutagenesis. Different M0 lines derived from different parental cultivars. A particular phenotype occured among most of the mutant lines. Now I don't know which cultivar was used to generate which mutant
line. I'm interested in determining those mutants derived from one type of cultivar
Hi Kingsley,
After mapping of the mutations, you could determine this by using molecular markers that:
- are near located around the mutation on both sides
- and are polymorphic between the concerned cultivars
regards
Stephan
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