I'm aware that a t-test could be used between means at each time-point; but isn't it wrong to analyse each time point separately (increase type 1 error?).

Do I have to include time in as a factor, in which case do I have to use an ANCOVA? Or a factorial ANCOVCA? I'm also reading of linear mixed effect modelling....

Any help figuring out the right test will be really appreciated! Many thanks!

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