To reach the Minimum effective concentration a specific dose of the drug is given to the human. Similarly, for laboratory animals like swiss albino mice, a specific dose of plant extract may be given. At what dose should I start administering to reach MEC?
Hi Sohel, the dose can be varied for various plant extract.In vitro antioxidative test can be done to fix a minimum effective dose or it will be very effective if you carry on your experiment with different concentrations of the extract.For example 50, 100 or 200 mg/ ml/ mice/day.
@Marzan.. thank you. But I was wondering that not all extract have the same minimum effective dose. Many journals start their dosing ranging from 200-400 mg/kg. What might be a safe range for finding pharmacological action
As far I see 100-300 mg/kg is safe dose.But it will be better if you run with your experiment more than one conc.of dose.Then there will be no risk of repeating your experiment.Moreover you will get better effect comparing two result.
I think the calculation for dose is dependent on LD50 and the dose generally select by LD50/10 or LD50/20.
So if LD50=4000mg/kg, then you can use 400mg/kg and 200mg/kg dose.
Perform the acute oral toxicity studies as indicated in OECD guideline 425. For edible variety of plants the limit test specifies 2000 mg/kg as the starting dose. Take 1/10 th of the dose as your starting dose and increase it logarithmically. N.B. Female wistar rats are preferred in this guideline.
The guidelines are well explained here:
https://ntp.niehs.nih.gov/iccvam/docs/acutetox_docs/udpproc/udpfin01/append/app
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