I want  to determine the quantum yeild of FITC in PBS buffer, I used Rhodamine 6G in ethanol as standard (quantum yield 0.95) and i excited at 480 nm and keeping the absorbance at 0.072. I use the same instrumental condition for the sample but the calculated quantum yeild was greater than 1 (2.19). I try various wavelenght and obtained the same result. How can i accurately determine the quantum yeild of FITC, what standrad is most appropriate

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