What speed and for how long do you spin the specimen to make a pellet for cell block?

08 August 2017 1 4K Report

Looking for ways to improve cell block for cytology.

Hello, in my lab we use HistoGel - Thermo Scientific (Ref. HG-4000-012). The first spin (sample +fixative are made at 2800rpm (aprox. 1300-1500g) for 5 min. The second spin (sample + histogel) 3500rpm (2000-2250g) for 1 min.

Hope it helps

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