I used MeOH and water for extracting natural products from bacteria but active fraction did not misible in water methanol solvents for HPLC analysis. How can I prepare HPLC sample for for analysis? Is there any alterative solvents?
Acetonitrile is aprotic, and should dissolve some non-polar analytes that MeOH will not. In the past, I have also used 50/50 tetrahydrofuran with either ethanol or acetonitrile for even less polar analytes.
Thanks. But my sample dont dissolved in Acetonitrile. I used 0.1% aqueous acetic acid, 0.1% acetonitrile and MeOH as a mobile phase in HPLC.
Try pH and DMSO or DMF. THink that if your simple is insoluble as you state, it will not run through the column (ie, you woll not have a separation of your analytes there).
Thank to every body for some good suggestions. Now I will try--------. Best wishes.
solvent for determine what? flavonoids or polyphenols or carbohydrate or .........
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